Chromatographic separation of reducing sugars in the urines of newborn babies.
نویسندگان
چکیده
The application of paper chromatography to the qualitative analysis of reducing sugars by Partridge (1946) has greatly simplified the detection of these substances in biological fluids. In particular it has provided an accurate and comparatively easy method of separating reducing sugars in the urine. This has proved of great value in the investigation of such a condition as congenital galactosaemia, about which disease there has been much recent interest (Bray, Isaac and Watkins, 1952; Hudson, Ireland, Ockenden and White-Jones, 1954; Cox and Pugh, 1954). It is now almost a routine procedure for the urine of a baby becoming ill with jaundice in the first few days of life and not thriving to be tested for reducing substances. Hudson and Ireland (1954) have, however, warned against reaching a hasty diagnosis of galactosaemia if galactose is found in the urine of such a baby. There is little information in the literature about the physiological excretion of reducing sugars by newborn babies. Woolf (1951) said that small amounts of galactose and lactose are found in the urines of milk-fed infants, but he gave no figures. He also said that xylose is frequently present in normal urines, but rarely exceeds 3 mg./100 ml. Rubin (1954) stated that sugars may be found in small amounts in the urines of healthy infants in the early months of life, but he gave no details. Flynn, Harper and de Mayo (1953) found lactose in the unnes of 900 of normal men and women. This paper is a report of our findings of the examination by paper chromatogaphy of the urines of 50 normal male babies.
منابع مشابه
Sugars in the blood and urine of children following the ingestion of disaccharides.
It has previously been shown that reducing sugars are frequently excreted in the urine by mature and premature newborn babies (Haworth and McCredie, 1956; Haworth and MacDonald, 1957). Other workers, using paper chromatographic methods, have also found small amounts of sugars in the urines of children and usually higher concentrations of lactose and galactose in urines from young babies (Woolf,...
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عنوان ژورنال:
- Archives of disease in childhood
دوره 31 157 شماره
صفحات -
تاریخ انتشار 1956